FastQCFastQC Report
Tue 21 Mar 2023
SRR9602569_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602569_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95652630
Sequences flagged as poor quality0
Sequence length150
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA17115631.7893527862223966No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4724180.4938891905010871No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2253480.235589967573291No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1107300.115762629840915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA5606900.0145.010511
CGACACA6664400.0143.392671
TAGCGAC5660300.0143.137072
ACACGAC4351550.0141.868551
GAACAGG9973000.0141.596771
AGCGACT2635200.0141.209733
AACATCG4009700.0140.672882
ATCCGTC2838650.0140.620562
AGCACCT4863600.0140.510391
ACGACCT2199350.0139.660133
AACAGGC10081250.0139.383292
AACGTGA2282400.0139.3491
GACACAC6832250.0139.144262
CACGACC4417900.0138.926482
GATGTAC2761200.0138.869722
AGTTAGC2638250.0138.716752
GCGACTA927600.0138.143694
ACATCGA1366200.0138.07093
AATCCGT2903400.0138.0611
AGCGACA1912000.0138.045323
Produced by FastQC (version 0.11.5)