FastQCFastQC Report
Tue 21 Mar 2023
SRR9602569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95652630
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG43283364.525056969160179No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA5391220.5636248579887453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2289750.23938181312944556No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1235550.1291705204551093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1028460.1075203055054524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT53260000.090.139851
AGTGGTA54458650.088.1311655
AGCAGTG54929500.087.372112
GTATCAA55121900.087.127199
GGTATCA55256150.086.895718
CAGTGGT55272800.086.8391954
GTGGTAT55377000.086.657156
TGGTATC55384100.086.6494147
GCAGTGG55904200.085.843183
CGATAGG290450.024.04357735-39
CATGGGG10733200.023.76183325-29
ATGGGAT5366150.023.28899825-29
ATGGGGT2446800.022.33966425-29
ATGGGAC3270500.022.14021725-29
ATGGGAG5707200.022.04792225-29
ATGGGGC3177000.021.90707625-29
ATGGGGG3304850.021.21862425-29
TACGCAC272500.020.59193450-54
CGACCTA272900.020.47670445-49
TGCGATA305100.020.04304330-34
Produced by FastQC (version 0.11.5)