FastQCFastQC Report
Tue 21 Mar 2023
SRR9602568_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602568_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95522124
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA50356525.271712760490963No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4048040.4237803589878299No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC3057710.32010490051498436No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2117320.22165755024459044No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCTATGTACTCTGCGTTGATA1312280.1373796922689868No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA982020.10280550294296219Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA4220000.0144.127331
TAGCGAC4223000.0142.710572
CGACACA5113550.0141.716691
GATGTAC8469400.0140.09582
TCACGCA3446550.0139.755632
AGATGTA8494950.0139.568151
TTCACGC3462050.0139.384611
CCGACAA2050100.0135.818121
CACGCAT1673550.0135.694583
AGTTAGC2673800.0135.213442
ATGCCTA2512750.0135.130721
AGCGACT2089000.0135.005873
GCGACTA707950.0134.686284
GACACAC5350650.0134.636372
GAGTTAG2709950.0134.131261
AGCGACA1484250.0133.30673
AACGTGA1333300.0132.970251
AGCGACG929250.0132.773443
ACCTTAC2454550.0132.379092
GGCTAAC1747950.0132.254782
Produced by FastQC (version 0.11.5)