FastQCFastQC Report
Tue 21 Mar 2023
SRR9602568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95522124
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG68125147.131870308913984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA6680990.6994180740788386No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1562960.16362282731485325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1399160.14647496741173804No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG1163790.12183460242152908No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1141880.11954089295585597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT65575000.074.8317641
GTATCAA67993350.072.227679
AGTGGTA68170300.071.9979555
GGTATCA68256500.071.933398
TGGTATC68967150.071.16087
GTGGTAT69009250.071.114016
AGCAGTG69200700.070.9072952
CAGTGGT69540450.070.579664
GCAGTGG70272650.069.81893
GACGCGT100200.052.9629251
ACGATAC92650.047.6145171
GATCGTA117950.046.5973431
TACGGTA108100.045.7252661
GTAACGT128150.043.797361
GCGCGAT92450.041.8118861
AGCGATA88700.041.555298
TACGGAT122750.041.157611
TCGCGAG131350.040.9568521
CGTCGAA57150.040.812497
CCGGTAG150950.039.641571
Produced by FastQC (version 0.11.5)