FastQCFastQC Report
Tue 21 Mar 2023
SRR9602567_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602567_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87768929
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA13995411.5945745447116029No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA5982450.6816136494043353No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2776280.3163169508425926No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2337910.26637102977524085No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2166060.2467912078544333No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1347870.15357029137270206No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1028650.1171997894608011No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC970660.11059266770818178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA4096300.0142.958191
TAGCGAC4126950.0141.173222
CAGATTC6859400.0136.756522
GTGCGAA1503950.0136.180332
TAGGATG3782150.0136.164541
TATCAGC2773500.0135.729061
GAACAGG6284150.0135.512821
ACAGATT6977550.0135.410551
TCACGCA2354050.0135.385162
AACATCG2139450.0135.014042
AACGTGA1469800.0134.44311
AGCGACT2040700.0134.121443
ATCCGTC1384450.0134.051792
AACAGGC6317850.0133.789662
GCGACTA686500.0133.445684
AGATTCG1181850.0133.347763
TTCACGC2398800.0133.267271
AGCGACA1385700.0132.37343
ACGTGAT1503350.0131.59162
GCACCTC3594950.0130.883132
Produced by FastQC (version 0.11.5)