FastQCFastQC Report
Tue 21 Mar 2023
SRR9602567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87768929
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG53409656.085257118723643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA9421571.0734516311575364No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1932190.22014510396953804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1439930.16405919684857953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1292180.14722522135367516No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG1091590.12437089211832585No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA979150.11155998041174685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT58024600.077.563721
AGTGGTA60044800.074.903445
AGCAGTG60280150.074.564322
CAGTGGT60813400.073.941154
GTATCAA60998000.073.868439
TGGTATC61227750.073.430297
GGTATCA61319400.073.357518
GTGGTAT61371300.073.280116
GCAGTGG61627950.072.908193
TCGCGAG99000.050.134061
ACGATAC83850.047.996081
TACGGTA86300.047.2238041
TACGGAT117050.046.630881
GATCGTA115950.046.5083771
CGCTATC129400.046.0047571
GTCGACT97050.045.9159938
GTACCGT129450.045.6572728
GACGCGT96400.045.0693241
CCGGTAG129500.043.7775461
AGCGATA81550.041.7547658
Produced by FastQC (version 0.11.5)