FastQCFastQC Report
Mon 20 Mar 2023
SRR7868838_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868838_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17928528
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTC2772751.5465575311035016No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGT2642531.47392468584147No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1717150.9577752283957723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG972270.5423033056590034No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC854450.4765868118118788No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA717920.4004344361121002No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA698650.38968620290522454No Hit
CCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCG465100.25941895508655255No Hit
CGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGG442400.24675756983506955No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCG421380.2350332386462514No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACC354070.19748972140936502No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCG300250.167470525187567No Hit
CCCGTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAG289090.16124580891415066No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACC282130.1573637277973964No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCAC233690.13034533565722742No Hit
GCCCCGTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACT231350.1290401532128014No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAAT197740.11029349425675103No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT192890.10758830842108175No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCG185050.10321538946197927No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTC183880.10256279823976625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCAG398400.027.2212491
GCGGGTC484450.023.8102437
ACCGACG23300.023.34359437
ACACCGA23300.022.78817635
GCGTGCG56800.021.92012637
CGCCCCA85550.021.5061381
AAACCGC25050.020.97737518
CCGCTAA22150.020.3714874
CAAACCG26500.020.17776917
ACCGCTA22150.020.1195583
ATATCGG29500.019.37849413
CGCTAAG23350.019.3249875
ACGTTCG64600.018.8664346
GCCCCGT56750.018.8004951
TCGTACG23650.018.77466414
TACGTTC39650.018.76090227
CATATCG30700.018.49952312
CAATCGG16100.018.4973059
TCAATCG18000.018.2910638
CGTTCGA66750.018.287637
Produced by FastQC (version 0.11.5)