FastQCFastQC Report
Mon 20 Mar 2023
SRR7868838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17928528
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGG1262900.7044080807972635No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG1158540.6461991748569653No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGG939870.5242315487361817No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT776610.4331699735750754No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGG741000.41330777406823366No Hit
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCG722590.40303922329819825No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGC665660.37128536151991953No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG634350.35382157419727933No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGT533190.29739753313824757No Hit
ATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGG402740.22463640071287505No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGG345770.19286022812358047No Hit
CTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGC268770.1499119169181095No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG250830.13990551817751018No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGT246820.1376688593731733No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG205000.11434290645612401No Hit
CGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCT204210.1139022679385614No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAAC203980.11377398077522037No Hit
CCTGTATCCTAGGCTACACACTGAGGACTCTGTTCCTCCCCTT190030.10599308543344999No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC187350.10449826109538943No Hit
CGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCA185150.10327116648951884No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGG183940.10259626445629001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC209650.023.0118521
CGCAAAA38650.021.4982071
AATACCA149900.019.3081461
CGACCGT34550.017.836171
ACCGTTG37100.016.705663
ATACCAC172850.016.6973192
GGCGATC16400.016.4702642
GACCGTT38350.015.9681342
TACCACT319250.015.9059121
GCAAAAC55300.015.5567672
ACGGACA39700.015.51678218
ACACGGA39750.015.49691816
GATCGCT88650.015.37973330
CCTAATA51450.015.28064815
TAACCCC51750.015.2647499
CGGACAC40250.015.25853613
ATCGCTT88800.015.2289731
GTCTAAC17750.015.2177224
ACCACTA336000.015.1971232
CGCGGTG50750.015.0962721
Produced by FastQC (version 0.11.5)