FastQCFastQC Report
Mon 20 Mar 2023
SRR7868837_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868837_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21288359
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2156241.012872809970933No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1265920.5946536320624808No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGT907680.4263738693997034No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT828190.3890342134872867No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTC493160.23165712303141825No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC236710.11119222482108648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCAG160200.024.1451661
TGACGGC28200.022.10728337
CCCGTCG30800.019.3902241
GTCAAAC215700.018.5372287
CCCCAGT211500.018.4974922
CGGAGCG216100.018.4582934
CCCAGTC214750.018.27143
CTGACGG35000.018.0769836
AGCGGGT221400.017.97286837
AACTCCC226500.017.61507211
CCGTCGG34550.017.6057852
CAGTCAA224650.017.4926245
CCGGAGC228600.017.48270833
CCAGTCA226750.017.3056664
GTCCCCG230100.017.21802329
TCCCCGG233550.017.04279130
GAGCGGG235350.016.96309536
GCGGGTC123450.016.90334337
CCCGGAG237200.016.84956232
GGAGCGG237800.016.78102335
Produced by FastQC (version 0.11.5)