FastQCFastQC Report
Mon 20 Mar 2023
SRR7868836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27292559
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG1344670.4926874024528078No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG860010.31510786511444383No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGC805480.29512806036253325No Hit
TACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGG407540.1493227513037528No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGG387580.14200940263608114No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGG308410.11300149612207487No Hit
CACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGG302740.11092400679613809No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCG301700.11054295055293276No Hit
AATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCG291270.10672139611386386No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCACTCA291190.10669208409515576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA52250.023.197721
ACACGTC31300.022.34131614
CGGTGGC248100.022.2045671
ACGTCAG34900.020.4607916
GATCGGA35300.020.2311672
CGTCAGA37300.018.89601917
CACACGT43000.016.5637413
CGTGTAG35650.016.29381236
ATCGGAA43850.016.1593513
GGCAATC41500.015.78011437
TGGCGCG353550.015.6045324
CCGGTCA79650.015.28291613
CAACCCG81400.015.2274949
GGTGGCG369500.015.1016772
GTGGCGC367150.015.0315743
CACGTCA48950.014.81476315
AGATCGG49950.014.7818121
TCGGAAG50700.014.2679894
CCCGGTC87650.014.24697912
ATACCAC92450.013.8686642
Produced by FastQC (version 0.11.5)