FastQCFastQC Report
Mon 20 Mar 2023
SRR7868835_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868835_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27938723
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5544051.9843605593569897No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2157710.7723008671513011No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC686240.2456232520004583No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA496330.17764949385839862No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGT467220.16723026317272985No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTC453490.16231593691665866No Hit
GTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTC383910.13741143430213326No Hit
GGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTT323620.11583206576764443No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTT288470.10325096104070325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCGC40850.020.83635534
CGGAGCG102950.019.75273934
GCTTTCG45500.019.0321633
TTCGCTC45900.019.0277636
AGCGGGT106400.019.02619237
CAATCGG20150.018.3611099
TCGTACG12150.017.20361314
GTCCCCG118950.017.1868829
CGTACGA12400.016.55835715
TTTCGCT52600.016.53365535
GTCAAAC125950.016.4058887
CAGTCAA123350.016.3756545
CCGGAGC126600.016.09189633
TACTCGA23650.015.72395422
ACTGTCC131200.015.62499426
CGCTAAG14050.015.4036885
AACTCCC136100.015.24949111
ACCGCTA13350.015.2406433
CCCCAGT135000.015.22133352
TGACGGC30900.015.21077737
Produced by FastQC (version 0.11.5)