FastQCFastQC Report
Mon 20 Mar 2023
SRR7868834_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868834_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26496377
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4792961.8089114598573233No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1931490.7289638126752197No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC1121840.4233937341697697No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA756700.2855862142963923No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGT444240.16766065790806042No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTC435850.16449418726190376No Hit
GTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTC360030.1358789543189244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA10900.021.04327615
TCGTACG11150.020.40605514
TGACGGC44850.019.80365837
CGTCGTG17750.018.2379113
ACCGCTA12700.017.4774993
GCGTCGT18550.017.3518112
TCGACCG7800.017.3144228
AGCGGGT109650.017.19618837
GATCGGA19300.017.0583972
CGGAGCG114150.016.68010134
AGAGCGT20100.016.5663419
CGCTAAG13700.016.202785
CCGCTAA14200.016.0231864
AGATCGG20350.015.9974521
CAATCGG17350.015.5669499
GTCAAAC124300.015.3439397
AGTCGTA15700.015.08168212
CTGACGG60300.015.00565136
CAGTCAA127550.014.7927375
GTCCCCG128600.014.76043629
Produced by FastQC (version 0.11.5)