FastQCFastQC Report
Mon 20 Mar 2023
SRR7868834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26496377
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG2005030.7567185506154295No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGC1386750.5233734408292877No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCC398540.15041301684377453No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGT362010.13662622629501384No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309670.1168725822402059No Hit
ACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTC265310.10013067069509164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC300900.026.991121
CGCAAAA49900.021.4340781
CGTGTAG38750.018.57084336
ACACGTC18550.018.5492714
ACTAAAT69750.018.1463531
GGTGGCG497100.016.6143472
GTGGCGC504950.016.3007013
TGGCGCG507750.016.1889174
AGCGCAA54950.016.0262054
ACGTGTA46750.015.51156835
GATCGGA22050.015.438752
GGCGCGT541750.015.204395
CGTCAGA23500.015.03537617
ACGTCAG23300.014.84702316
CGCAAGT60900.014.4916796
ACCGTTG28100.014.4188313
GCGCGTG572950.014.3830546
GACCGTT28700.014.3755972
GCGTGCC592400.013.881568
CGCGTGC593800.013.8487817
Produced by FastQC (version 0.11.5)