FastQCFastQC Report
Mon 20 Mar 2023
SRR7868832_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868832_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26559810
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9348333.5197277390162056No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7714942.904742164947716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1881010.7082166626944997No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC993010.37387692155930335No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA692950.2609017157878765No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTC645920.24319451080410592No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGC583360.21964012543764433No Hit
CACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG542710.20433504607148922No Hit
GGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTT338120.12730512755927093No Hit
GTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTC328310.12361157704064903No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267650.10077255823742716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC89600.026.59955637
CTGACGG107450.022.40444236
GCGTCAG37500.019.88567437
ACGTTCG9500.017.1351596
CACTCCC134850.016.7898371
CAATCGG18150.016.3075549
TATGGCG47400.015.92698833
GGGGCAA183050.015.6327921
CGGAGCG39250.015.60429834
AGCGGGT41050.015.2359737
TCGACCG6000.015.10858528
CGTCTGA74300.014.99239336
CGATTGA28400.014.7200534
TACGTTC16600.014.71122627
GGCGTCA50850.014.62845136
ATATCGG37100.014.45927813
GGAGGTC327500.014.28907437
GAGGTCA219000.014.21177837
CCTACGT17200.014.19868825
GCTGACG170650.014.08519535
Produced by FastQC (version 0.11.5)