FastQCFastQC Report
Mon 20 Mar 2023
SRR7868831_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868831_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26758619
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9060263.3859221210182784No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6720212.5114188441488703No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1863660.6964709202668493No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC828710.30969834429796245No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA571570.21360220420941753No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTC383550.1433369935870009No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAAC281140.10506521281983947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC44000.017.83199337
CGTACGA11050.017.74436815
TCGTACG11950.016.56282614
ACCGCTA13150.016.3164483
CAATCGG18000.015.7239869
CTGACGG52300.015.42575236
CCGCTAA15150.014.6516184
CGGAGCG52600.014.63401234
TATGGCG39300.014.40720833
TTACGCG1803.3451215E-914.38761111
GCGTCAG39150.014.32179837
AGCGGGT52550.014.29683837
GGAGGTC287200.013.94310637
CGCTAAG15000.013.9350395
TACGTTC14800.013.7505827
ACGTTCG12450.013.6695726
GTCGTAC15050.013.39745613
GCCCCGT36900.013.38361551
ATATCGG37650.013.36399513
GCGATTG32200.013.2693463
Produced by FastQC (version 0.11.5)