FastQCFastQC Report
Mon 20 Mar 2023
SRR7868830_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868830_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22039643
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4324871.9623139993692276No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2324201.054554286564442No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG994920.4514229200536506No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC933950.42375913257760117No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA623200.2827632008376905No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGC468080.21238093557141555No Hit
CACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG459560.20851517422491828No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAAC408040.18513911500290636No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTC400410.18167717144964643No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACC329980.14972111844098382No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCA275510.1250065620391401No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGG266620.12097292138534185No Hit
GTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTC246070.11164881391227616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC77300.023.79419537
CTGACGG91700.020.23894336
GCGTCGT13100.016.94566712
TACGTTC20800.016.4549527
CCCTACG21800.016.04080224
AGAGCGT14200.015.76314459
CTACGTT21900.015.71374526
CACTCCC119400.015.6603661
ACGTTCG12850.015.402916
CAATCGG18550.015.35757359
CCTACGT22600.015.30921625
ACCGCTA12350.015.2762553
ACCGACG8750.015.01462337
GCCCCGT33550.014.9944831
CGTACGA12400.014.76911215
CGTCGTG15300.014.50910313
GGAGGTC312850.014.39622337
GATCGGA16200.014.3847642
CGGAGCG47350.014.18534634
CGATTGA39900.013.9079654
Produced by FastQC (version 0.11.5)