FastQCFastQC Report
Mon 20 Mar 2023
SRR7868830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22039643
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG2340621.0620044979857433No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2229521.0115953330097045No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGC1525050.6919576691872913No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA921320.4180285497364908No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGT528870.23996305203310234No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCC476750.2163147560965484No Hit
TGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATA303980.13792419414416104No Hit
ACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTC278350.1262951491546392No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCT272800.12377695954512512No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGC266990.1211408006926428No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGG250850.11381763307146127No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCACTCA224010.10163957737427962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC325100.029.4552881
CGTGTAG52000.020.70481336
CGCAAAA51150.019.426681
ACTAAAT63450.019.3645021
ACACGTC14000.018.49931114
AGTCACG14550.017.67312429
AGCGCAA65400.017.5959034
TGCAGCG68300.017.3392051
GGTGGCG564300.017.2985382
ACGTGTA63500.017.01338835
GTGGCGC587850.016.6018283
CGCAAGT70550.016.6008876
TGGCGCG592400.016.5023844
CTAAATA123750.016.1790081
CAGCGCA81550.016.0850433
GGCGATC21300.015.9828022
GTGTAGA69100.015.768517537
GGCGCGT620950.015.7144145
GCGCAAG75600.015.5650325
AACGTGT70100.015.54343234
Produced by FastQC (version 0.11.5)