FastQCFastQC Report
Mon 20 Mar 2023
SRR7868828_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868828_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13784855
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2363881.7148384948554047No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1708791.2396140546998864No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1045380.7583540051745195No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGT347480.2520737432493849No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTC339260.24611067726138577No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC299150.2170135268016965No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA209790.15218876078130672No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTC197760.14346179194485542No Hit
GGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTT161870.11742597219919976No Hit
GTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTC155370.11271065237900581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG13250.018.85357737
ACCGCTA15650.017.965253
GCGTCAG33900.017.95858437
ACACCGA13650.017.89407535
AGCGGGT76400.017.85048537
TCGAACC13550.017.61363826
CGTACGA15700.017.3190815
TATGGCG36750.017.16941833
GCCCCAG82450.016.6895751
TCGTACG16450.016.64196214
CAATCGG12700.016.6052789
AAACCGC14650.016.54176518
CAAACCG15000.016.5253517
CGGAGCG83300.016.48264534
GGCGTCA38100.016.0272936
CCCCAGT86850.015.9293472
AACTCCC86550.015.85864511
AGTCGTA17450.015.79547612
TGACGGC20800.015.74656537
GTCAAAC86900.015.6890797
Produced by FastQC (version 0.11.5)