FastQCFastQC Report
Mon 20 Mar 2023
SRR7868828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13784855
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940151.407450422946052No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG430400.3122267154786902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA392650.2848415888306406No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCC356440.2585736302630677No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGC324820.23563541292237022No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGC219340.15911665374789943No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCACTCA199780.144927168258208No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGC188030.1364033208909343No Hit
TGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATA177430.12871372241492565No Hit
ACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTC176370.1279447625673248No Hit
CCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTC156620.11361744465212002No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATA154810.11230440944065062No Hit
CTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCC143120.10382408810248639No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGT138440.10042905783194674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA33900.024.3462031
CGGTGGC88400.021.1010651
AGCGCAA35950.018.5792084
ACTAAAT45800.017.6972061
CGACCGT19050.017.4853211
TAAATAC86050.016.8794442
CAGCGCA40950.016.7625033
CTAAATA88050.016.3300571
CGCAAGT41500.016.229156
TGCAGCG42650.016.0972211
CCGTCAT51000.016.0379241
GACCGTT20750.015.9615212
TTAACCC50800.015.9144818
ACTACCG92450.015.7884157
TACCGTA92250.015.7229459
ACCGTTG21100.015.6960173
CTACCGT92300.015.6739998
CCGTATG92900.015.47347811
CGTATGG92900.015.47291812
TAACCCC52700.015.2006269
Produced by FastQC (version 0.11.5)