FastQCFastQC Report
Mon 20 Mar 2023
SRR7868827_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7868827_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8065218
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1252211.552605273657823No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG394130.4886786693180519No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA354810.43992611235058987No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTC188670.23393044056589668No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC175490.21758866282349715No Hit
GGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTT111800.13861993562976227No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA106860.13249486870658675No Hit
GTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTC89310.1107347625321473No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGG88540.10978004562306934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT6300.018.4986539
GATCGGA6500.018.4948672
CGATTGA15100.018.1288814
AAGAGCG6750.017.53998
AGATCGG6900.017.157321
GCGTCGT7100.016.93475712
CAATCGG8000.016.8800229
GCGTCAG18000.016.7584237
CGTCGTG7200.016.44243213
ACGTATA4050.015.99035832
CGTCAAG8800.015.98116636
AGCGTCG7750.015.75310611
ACCGACG8250.015.70225537
ATCGGGA9000.015.62050311
ACGTCAA8800.015.56041535
CGGTTGA17550.015.49575119
CGCCGTA22500.015.46039332
TCGATTG8750.015.43574925
CAACGTC9150.015.36948333
GTCAACG8800.015.34794931
Produced by FastQC (version 0.11.5)