FastQCFastQC Report
Mon 20 Mar 2023
SRR6503041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110546024
Sequences flagged as poor quality0
Sequence length150
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1224697611.078621877888615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA12416311.1231801516443505No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7611260.6885150387679253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA6119490.5535694345732417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA5516730.49904372861026647No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4719740.42694796512989014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4165090.37677429266926865No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA2522000.22814027214583493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2122480.19199966884381117Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT2071420.1873807781634914No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1915280.17325634434396303No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGGAAGAGCACACGTCTG1713280.15498341215781763Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1551680.14036506640890134No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC1411970.12772689138055296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAAAAAA1359050.12293974498802417No Hit
GAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1266450.1145631434017021No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTTGAGTGCTGAAAAAA1214680.10988002607854987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC1133020.10249305755221012Illumina Multiplexing Index Sequencing Primer (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT117968750.088.7377241
AGCAGTG120993600.087.594122
AGTGGTA121640250.087.1545945
GTATCAA121460300.087.0533149
GGTATCA122126000.086.5632258
CAGTGGT122561950.086.436174
TGGTATC122525950.086.229617
GTGGTAT122859450.086.186356
GCAGTGG123438100.085.8733
GAGCAGT1658750.064.5218051
TGGTATA631100.029.1934037
GGTATAA590550.029.0035368
CAGCAGT1245200.025.8089051
CATGGGG24738100.025.28351825-29
ATGGGGT6350400.025.1849425-29
ATGGGGG7936350.024.74750525-29
ATGGGGA8163900.023.43738225-29
ATGGGGC3746650.023.2121225-29
CATGGGT12564850.022.64654725-29
ATGGGTA3359450.022.55097825-29
Produced by FastQC (version 0.11.5)