FastQCFastQC Report
Mon 20 Mar 2023
SRR6503039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163357662
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6232747238.15399365840581No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA73134674.47696600848756No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA12003350.7347895319412688No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA9795850.5996565989050456No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT9462080.5792247442914554No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9296840.5691095162711132No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8934170.5469085374152821No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT8299820.5080765663749521No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC6938600.4247489781042532No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6899010.42232546153849826No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG6835170.41841747220892517No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA6825920.41785123002066477No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5799990.3550485437285458No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5780650.35386463843979354No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA5395790.33030529048585433No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG5391930.33006899915107746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG5171540.31657774338126854No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA5153530.31547525453688235No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG4825990.29542477168900716No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA4426200.2709514782355296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA4402460.2694982253112805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC4338660.2655926845965756Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4330600.26509928870064264No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4297830.2630932609699078No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA4233060.25912834134465024No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3940150.24119774681887896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3601540.22046960980624222No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT3573350.21874394847791098No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3194840.1955733181342911No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA3170590.19408884537047305TruSeq Adapter, Index 2 (95% over 22bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3068980.18786875145164603No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2842600.17401081560533108No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA2801210.17147711137050922No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA2764140.1692078575414479No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG2763390.16916194601267004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA2721950.166625181009263No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2686560.16445876900466413No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2674480.16371928731448177No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA2642730.1617756992628849No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG2511080.15371669557807457No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2468150.1510887196708288No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCGG2467250.15103362583629534No Hit
AAGCAGTGGTATCAACGCAGAGCACACGTCTGAACTCCAGTCACTGACCA2388740.14622760700382698TruSeq Adapter, Index 4 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC2205620.13501784813742007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAAAAAA2034700.12455491680580004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2034070.1245163511216266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCAGTGGTATCAACGCAG1840170.11264669054825234No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT1801050.11025194520719818No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGGAAGAGCACACGTCT1693860.10369026951426374Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC1693620.10367557782505481Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1691710.10355865646510048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGGTGGTATCAACGCAG1688950.1033897020391979No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAG1638050.10027383961947252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT374004100.045.7582471
AGCAGTG392206100.043.6456262
AGTGGTA393810150.043.5017975
GTATCAA396899450.043.1902059
GGTATCA405966100.042.2100648
CAGTGGT406868400.042.094134
GCAGTGG407479000.042.0181853
TGGTATC414691050.041.3164947
GTGGTAT415243850.041.2647366
GGGGAAA3088950.017.35325425-29
TTAGGGC339300.016.29909745-49
GGGGGAA1842750.015.9601525-29
GGAATTC613950.015.47535330-34
CCTAACT372050.015.34375960-64
TGGGACT981050.015.1705825-29
GGGCTAA408000.015.12131225-29
TGGGACA778350.014.78357125-29
TGGGAAC1413650.014.65709425-29
ATGGGCT3325100.014.63560825-29
TGGGGAA3675750.014.53361225-29
Produced by FastQC (version 0.11.5)