FastQCFastQC Report
Mon 20 Mar 2023
SRR6503037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114861408
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2372168920.652444901250036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA53029344.6168108961366725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA24606162.142247812250395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA16323821.4211753350611895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15189381.3224093509283814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT5158640.44911864566382464No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4887720.42553195935052446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA4878590.42473708837001195No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA4690750.40838346679504395No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3870390.33696174088341313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA3381130.29436605896386014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC3285420.28603340819224504No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3020200.2629429721077422No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC3002390.26139240779635925No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2893270.25189226306541534No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2845540.24773682036006384No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA2470740.2151061912805387No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2367290.20609968493508282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2296740.19995750008566845No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2176240.18946659612600256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA2063730.17967131310108964No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1993290.1735387050104766No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1934570.16842645703942616No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1819220.15838391951455097No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1716330.14942616757753832No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1701100.14810022179076893No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAAAAAAAAAAAAAA1681050.1463546398456129No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1626410.14159760256464904No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1546210.13461527478402494No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1525980.13285402177901215No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1490470.12976246991504753No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGGAAGAGCACACGTCT1415620.12324592085794386Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC1410500.12280016626646262Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1325710.11541822645949108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1323740.11524671541550317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCACTATAGGAAAAAATAAA1320140.11493329421836793No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1318120.11475743010219759No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAAAAAAAAAAAAAAAA1277920.11125756006752069No Hit
AAGCAGTGGGATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1260350.10972789050261338No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1254590.10922641658719698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT163702550.068.530311
GTATCAA169175650.066.1145559
AGTGGTA169568300.065.640765
AGCAGTG171353750.065.4675752
GGTATCA172373350.064.8573768
GCAGTGG175700450.063.813593
CAGTGGT174541200.063.756334
TGGTATC175759250.063.5923277
GTGGTAT175854700.063.523476
GGATCAA1508750.061.1495329
GTGGGAT1696150.054.13466
CAGTGGG1712700.052.981864
AGTGGGA1998300.045.2143025
GGGATCA2078300.044.3398938
AAGCTGT1322300.032.8394741
TGGGATC3319050.027.751447
TGTGGTA1541550.026.8583015
AGCTGTG1669750.026.0283322
TGGGGAA8887550.022.66038525-29
GGGGAAA11575150.022.5483225-29
Produced by FastQC (version 0.11.5)