FastQCFastQC Report
Mon 20 Mar 2023
SRR6503034_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503034_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110516555
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC14455661.3080085603464566No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6176950.5589162637217564No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2955460.26742237848438183No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2524550.22843183991755805No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1362280.12326479051034481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT4432450.0144.134831
CAATGGA9837550.0142.847841
ACGTATC1890350.0142.831571
GAACTTA4507000.0141.443762
AACGTGA1442600.0141.003481
CTCAATG3230700.0140.292481
ACTTCGA1387950.0140.222142
AACCGAG4269000.0139.955841
ATGCCTA1052300.0139.903611
CGCATAC2248650.0139.359591
CCGTGAG2754600.0139.338331
ACGCTTA1456350.0139.173222
ACGTGAT1483700.0138.849612
ACTAGTA2427550.0138.835722
CCGAGAT2351000.0138.781073
ACAAGCT1437850.0138.718481
CAAGCTA1449850.0138.337232
GACTAGT2445700.0138.055591
TCAATGA3261750.0137.883472
ACCGAGA4316350.0137.748282
Produced by FastQC (version 0.11.5)