FastQCFastQC Report
Mon 20 Mar 2023
SRR6503032_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503032_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93824958
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC9968121.06241667595524No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4795570.5111188006074034No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1470360.15671309972768654No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1128170.12024199360686097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC8586850.0144.74061
CGACTGG8902500.0144.235351
CTCAATG4725050.0143.606381
ACGTATC1468700.0143.368851
CTGGCAT3198850.0143.046971
GCATACA8751150.0142.635992
ACGCTTA3422000.0142.288072
AGTCACT1401650.0142.04111
ACTTCGA1595650.0141.774752
GAGCTGA2012050.0141.735461
ATGCCTA899900.0141.5311
CATACAT4266600.0141.26273
ACTGGAT4553350.0141.2473
AGTGGTC1965000.0141.013991
GAATCTG1504100.0140.798551
GTCACTA1432650.0140.567892
GACTGGA9135550.0140.550172
TCAATGA4839350.0140.486592
CACTTCG1614050.0140.220731
CAATGGA3408000.0140.099551
Produced by FastQC (version 0.11.5)