FastQCFastQC Report
Mon 20 Mar 2023
SRR6503031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101302941
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG79531427.850850055774787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA74371817.341525257396031No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA31463413.1058733033229506No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA27843812.7485687705749826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14425841.4240297327596836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA9240310.9121462722390261No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA6542370.6458223162543721No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA5884510.5808824444692084No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA4181650.41278663370691276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA4136490.40832871772202545No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2802180.2766138842898944No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA2623340.2589599052213104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAAAAAAAAAAAAAA2466230.24345097740054752No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2201000.21726911166379662No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2119440.20921801273272017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1962170.1936932907012048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAAAAAAAAAAAAAAAA1939010.1914070786947834No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1843990.1820272917841546No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAGAAAAAAAAAAAAAAAAA1369980.13523595529176197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1358910.1341431933353248No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1317880.13009296541548582No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAGGAAAAAAAAAAAAAAAAAAA1308440.1291611069810895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGAAAAAAAAAAAAAAAA1306170.1289370266160387No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT1131880.11173219541572835No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1037320.10239781686101296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT83808750.094.3708041
AGCAGTG86393300.091.486352
AGTGGTA86154700.091.0943155
GTATCAA86945600.090.687029
GGTATCA87863100.089.65928
CAGTGGT87552400.089.6413044
GCAGTGG88310750.089.44883
GTGGTAT89353750.088.139226
TGGTATC89397900.088.1192557
GTGGGAT945650.060.2741936
GGATCAA961650.059.4148039
CAGTGGG1055100.054.286964
GGGATCA1274500.044.790558
CGAGTAA96800.034.4565052
TGGGATC1811350.031.4832127
CTAACGA110100.030.2941842
GCTAACG113750.029.9681151
AAGCTGT1063300.028.5131021
TGTGGTA1031150.027.9624635
TAGCGAC75150.026.7448882
Produced by FastQC (version 0.11.5)