FastQCFastQC Report
Mon 20 Mar 2023
SRR6503030_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503030_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93309319
Sequences flagged as poor quality0
Sequence length150
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5942570.6368677923798801No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2178760.2334986497972405No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1132800.12140266504356333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTCA5386350.0143.552611
AGTCACT4183650.0142.490891
GAGTCAA5452300.0142.278952
ATGCCTA2270150.0142.248731
GTCACTA4214300.0142.20162
ACTTCGA2371950.0141.801832
CTGAGCC3790600.0141.442381
ACGTGAT2444250.0141.380552
TGAGCCA3758150.0141.283362
GACGGAT2734100.0141.115053
AGTCAAT3360400.0140.816593
ACGCTCG2107200.0140.58991
CAAGCTA1863700.0140.586942
CGAACTT1779000.0140.586441
CGCTCGA2113000.0140.48082
CTCAATG2055150.0140.248611
ACGCTTA1454250.0140.009922
AACGTGA2443200.0140.001531
CACTTCG2425950.0139.974671
GAGCCAT2237250.0139.953123
Produced by FastQC (version 0.11.5)