FastQCFastQC Report
Mon 20 Mar 2023
SRR6503029_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503029_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92048994
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA37939044.121613757125906No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8248670.8961173437702099No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7210180.7832980771088058No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA5550920.6030397246927001No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3914630.425276782492593No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1973200.21436410266471786No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1773500.1926691344394269Illumina Single End PCR Primer 1 (100% over 21bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1329990.14448718472686403No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1098160.11930168405751397No Hit
CCATGTACTCTGCGTTGATAGCACTGCTTCCCATGTACTCTGCGTTGATA1002510.1089104786957259No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA992040.1077730409525171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1521200.0142.244451
ATGAATC3138850.0141.614612
TATCAGC1861350.0141.255261
GATAGAC1417200.0141.198291
TAGCGAC1533350.0141.16182
ATCCGTC2255500.0140.34042
ATCAGCA1876300.0139.454682
TAGGATG3095150.0139.404371
AATCCGT2273100.0139.283481
GGCTAAC1686800.0138.826542
AGGATGA3096000.0138.812562
ATAGACA1448100.0138.674792
GTCGTAG1572250.0138.462841
TCTTCAC5998550.0138.400181
CTTCACA5987400.0138.372912
TGAATCA1056750.0137.287263
AGCGACT754400.0137.26043
GTGCGAA1322400.0137.083792
TAAGGTC1145250.0136.732032
TCACGCA938100.0136.6512
Produced by FastQC (version 0.11.5)