FastQCFastQC Report
Mon 20 Mar 2023
SRR6503028_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503028_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130035358
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12501150.961365446465722No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3998210.3074709880062006No Hit
AACCGAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1619470.12454074221874331No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1312130.10090563214352821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAG20483850.0143.152481
CCGAAGT5430300.0142.636281
ACCGAGA20490700.0142.309512
CCGAGAT11331550.0142.196463
ACGTATC2024600.0141.736771
CGAACTT3154950.0141.611681
ACGCTTA4220050.0141.536712
CGAAGTA5519250.0141.243592
ACAAGCT2973950.0141.063671
CAAGCTA2979150.0141.020072
GAACTTA3195750.0140.202942
CATCAAG1672100.0140.11151
GTCACTA2612300.0140.09312
CGAGATA3874700.0139.98694
GCTCGAT1583300.0139.68873
CGCTCGA2754050.0139.673142
CGAGATT5952150.0139.440774
AACGTGA1619350.0139.418081
ACGTGAT1629150.0139.346652
ACGCTCG2767400.0139.30211
Produced by FastQC (version 0.11.5)