FastQCFastQC Report
Mon 20 Mar 2023
SRR6503027_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503027_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84923195
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA39507434.652136557038392No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8437520.9935471692980934No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6950930.8184960539932583No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA5289400.6228451484897618No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3362970.39600135157420774No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1834240.21598810548755262No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1491760.1756599006902649Illumina Single End PCR Primer 1 (100% over 21bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1049730.12360933900331941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC2226400.0143.725111
TATCAGC1774550.0142.733631
AGTTAGC2337100.0142.24382
ATGAATC2922950.0142.00092
TTCACGC2564150.0141.59771
AGCACCT1696700.0140.988281
TCACGCA2573900.0140.734882
ATAGACA2280900.0140.726612
GCGAGTA1707450.0140.100631
GTGCGAA2788350.0140.081792
ATCAGCA1817500.0139.580692
GAGTTAG2385100.0139.355421
CGAGTAA1720050.0138.656772
GGCTTCA2437100.0138.611052
CCTAATC1170400.0138.453451
TGGAACA1760500.0138.445391
ATCCGTC1051250.0138.316022
CACGCAT1265350.0138.310353
GTCGTAG1076550.0138.189931
ACCTTAC1483650.0138.12672
Produced by FastQC (version 0.11.5)