FastQCFastQC Report
Mon 20 Mar 2023
SRR6503026_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503026_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91611429
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10995941.200280371131423No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2679570.29249298141610697No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1235630.13487727606563152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC3085050.0144.5131
GACTAGT8822250.0143.979771
CGAACTT2640150.0143.802141
CCGTGAG5923650.0143.572221
CCGAAGT2299250.0142.751541
CGCATAC3016800.0142.524191
CTCAATG3240200.0142.338211
ACTAGTA8944550.0142.244432
GAGCTGA3544750.0142.203641
CAATGGA5831900.0141.929691
CGTGAGA6028500.0141.622542
GAATCTG2346700.0141.458761
AGTCACT1157200.0141.067441
CTAGTAT4617000.0141.049033
GAACTTA2700250.0141.021152
ATGCCTA1013300.0140.8921
AGCTGAA3533650.0140.840712
ACTTCGA1268550.0140.778722
CTAGTAA3045650.0140.610573
AGAGTCA1137800.0140.429351
Produced by FastQC (version 0.11.5)