FastQCFastQC Report
Mon 20 Mar 2023
SRR6503025_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503025_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109222668
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA14622991.3388237320846255No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4941150.4523923550375093No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4785090.4381041122342845No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3331700.3050374121972556No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1382460.12657262684702045No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1153760.10563374994648547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA3407550.0143.230221
TAGCGAC3438150.0142.427372
TATCAGC3034250.0142.425461
ATGAATC4792900.0141.985032
AGTTAGC2885600.0141.604612
GATAGAC1919350.0141.357571
ATCAGCA3058050.0140.915852
TAAGGTC3521250.0140.81742
GGCTAAC4071500.0139.973662
AGCGACT1688700.0139.446333
TCACGCA1912800.0138.93062
ATCCGTC1717200.0138.86612
GACAACT1991200.0138.399143
ATAGACA1964450.0138.255722
GCTAACT2059700.0138.058073
CGGATTG2768400.0137.943731
CCGACAA3908650.0137.932631
TGAATCA1610250.0137.915763
TCAGCAT1704000.0137.907143
GCGAGTA1801000.0137.733871
Produced by FastQC (version 0.11.5)