FastQCFastQC Report
Mon 20 Mar 2023
SRR6503024_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503024_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114920974
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12376651.0769705101872875No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2988990.26009090385885525No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1195550.10403235879292147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAAGT4899600.0142.556661
ATGCCTA3147250.0142.359631
AGTCACT4265200.0142.312531
ACGTATC2579250.0142.250661
GTCACTA4256350.0142.169662
CGAACTT3191750.0141.414461
CGAAGTA4945400.0141.40272
AGTACAA5392500.0141.393451
CTGGCAT3025500.0141.350131
ACTTCGA2085150.0140.801732
TGCCTAA3181700.0140.134872
CAAGACT2518350.0139.97521
GAACTTA3222650.0139.942322
CAAGCTA1916550.0139.542972
ATCGCAT1602800.0139.462813
GATCGCA2883600.0139.214432
AGATCGC2909100.0139.200331
AAGACTA2541600.0139.045012
AGCAGGA4405650.0138.921041
GTACAAG5448350.0138.707722
Produced by FastQC (version 0.11.5)