FastQCFastQC Report
Mon 20 Mar 2023
SRR6503022_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503022_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93540884
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC18865112.016776963536073No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5031020.5378418275371442No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1826370.19524831516452207No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1592390.1702346537584571No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1518260.16230977676028804No Hit
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1340690.14332663351781025No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1039800.11115995012405484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT4329900.0144.679261
CCGAAGT3282850.0143.976461
ACGTATC2066800.0143.676481
ATGCCTA1794350.0143.437521
CACTTCG4114350.0143.095121
CCGTGAG3578250.0143.047931
CAAGACT3535350.0142.719421
ACTTCGA4123900.0142.605062
GAGCTGA2430700.0142.435621
GAACTTA4413500.0141.996462
ACAAGCT1430650.0141.124511
AGTCACT1223450.0141.068651
AAGACTA3584000.0140.971682
CTCAATG1796950.0140.770391
CGTGAGA3632300.0140.688492
AGACTAT1815250.0140.422843
GTCACTA1235150.0140.238432
AGCTGAA2470000.0140.092272
CGAAGTA3389550.0140.00552
CAAGCTA1445850.0139.816132
Produced by FastQC (version 0.11.5)