FastQCFastQC Report
Mon 20 Mar 2023
SRR6503021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78947169
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1364193717.279830515518547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA14021131.7760142862120871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10347951.3107436442717788No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA6301470.7981882162234342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4315680.5466541808484608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4135940.5238870566720385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3715310.4706071220869237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT3710530.4700016538908444No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3133150.39686666915187296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2921410.3700462014033714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC2606900.3302081674391643No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1946860.24660288958556578No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1852450.23464425937806585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC1821300.23069858274462002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT1489190.18863120981576934No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1432340.18143019162599738No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1417260.17952005346765507No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1405380.17801524966652066No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA1337530.1694208946238465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1300990.16479248293247856No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1261870.15983727041561174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCACTATAGGAAAAAATAAA1203210.15240698498004407No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1187570.15042591330919036No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1155250.14633203630139036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1137150.14403936384343308No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1124540.1424420931420606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTTCGTTTGTTCAACGAT1082160.13707394624878824No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1002500.12698365409404358No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT966690.1224477093029137No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC890590.11280835162056285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA880960.11158854853934028No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA849720.10763147187709797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC848450.10747060480408106Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG843390.10682966985174605No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG842740.10674733631043819No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG818790.10371366198071016No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA807010.1022215248782385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCATTATTACCCTCACTGTC800630.10141338950355522No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT798370.10112712211377713No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT792000.1003202534089601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT99504850.072.859241
AGCAGTG104203500.069.5999452
GTATCAA104038050.069.561859
AGTGGTA103878100.069.317845
GGTATCA106216950.068.1060648
CAGTGGT106986650.067.313154
GCAGTGG107919750.067.184233
TGGTATC109096150.066.3046957
GTGGTAT109181050.066.2159356
GGATCAA929150.059.6197249
GTGGGAT1057250.052.14686
CAGTGGG1083700.050.475874
GGGATCA1298550.042.58218
AGTGGGA1537450.035.588175
AAGCTGT899550.033.7448231
TGGGATC2179150.025.3712647
AGCTGTG1326950.022.9409732
TGTGGTA1330950.021.977495
ACCTAGC719600.021.931135-39
AAACCGC612700.021.70758285-89
Produced by FastQC (version 0.11.5)