FastQCFastQC Report
Mon 20 Mar 2023
SRR6503020_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503020_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84775535
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12787651.5084127749827825No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5449970.6428706111969685No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1699530.2004741108386989No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1349470.15918153745653152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA7663300.0143.040441
ACGTATC1095200.0142.79591
CAAGACT2276550.0142.63011
CGAACTT1632550.0142.451231
ACAAGCT1831550.0142.024171
AACGTGA1226550.0141.571911
GTCACTA1757950.0140.995162
AAGACTA2316500.0140.719382
CAAGCTA1865850.0140.709142
AGAGTCA953950.0140.654071
AGTCACT1735850.0140.60371
ACGTGAT1268900.0139.971852
CATCAAG889850.0139.937641
ACTTCGA937550.0139.857592
AGACTAT1202500.0139.57473
GAACTTA1672550.0139.361572
AGTACAA1350950.0139.221591
CTCAATG1650950.0139.032491
GAGCTGA1374750.0138.516371
GAGTCAA979950.0138.221682
Produced by FastQC (version 0.11.5)