FastQCFastQC Report
Mon 20 Mar 2023
SRR6503019_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503019_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102599402
Sequences flagged as poor quality0
Sequence length150
%GC19

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA8043890.784009442862055No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4027260.39252275563945294No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2213150.21570788492509926No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1907750.18594162956232435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTC12688250.0143.240072
GGCTAAC4789450.0143.004362
ATGAATC3268900.0142.750082
CTAAGGT12727700.0142.681991
AGTTAGC3303500.0142.643362
GCCAAGA4422900.0142.516281
GGATTGC7200100.0142.424562
CGGATTG7210100.0141.903491
AAGGTCA4012500.0141.892823
CCAAGAC4484600.0141.687182
GCTAACT2386300.0141.671943
ACCTTAC3138750.0141.52352
AAGGTCG3323200.0141.509663
ATAGCGA935750.0141.494061
GAACAGG3972900.0141.273031
AACAGGC4006950.0141.198762
GATAGAC813250.0141.197241
TGGAACA2228550.0141.126651
CGACACA2334650.0141.051471
AAGGTCT5577350.0140.906853
Produced by FastQC (version 0.11.5)