FastQCFastQC Report
Mon 20 Mar 2023
SRR6503019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102599402
Sequences flagged as poor quality0
Sequence length150
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG83441588.132755003776728No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA51890995.057630842721676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA20105631.9596244820218347No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA19549551.9054253357149196No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17356481.6916745772066002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAA8282510.8072668883586671No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA7604080.7411427212801883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA7060990.6882096642239688No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA6704790.6534921129462333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGAAAAAAAAAAAAAAAAA6242600.6084440921010437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA5271300.513774924341177No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGAAAAAAAAAAAAAAAA4315860.4206515745579102No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4300830.419186653738976No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA3841060.37437450171493203No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA3833890.37367566723244644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGAAAAAAAAAAAAAAAAA3676780.3583627124844256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAAAAAAAAAAAAAAAA3499580.34109165665507485No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAAAAAAAAAAAAAAAAA3441040.3353859703782679No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAAAAAAAAAAAAAAAAA3294810.3211334506608528No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAATGAGAAAAAAAAAAAAA3261420.31787904572777137No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGAAAAAAAAAAAAAAAA2821320.2749840588739494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAAAAAAAAAAAAAAAAA2733410.2664157828132371No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAAAAAAAAAAAAAAA2686490.2618426567437498No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA2657890.25905511613020904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCAAAAAAAAAAAAAAAAA2349130.22896137347857057No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATGAAAAAAAAAAAAAAAA2173590.21185211196455123No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAAAAAAAAAAAAAAAAAA2075410.2022828554107947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGAAAAAAAAAAAAAAAA2068360.20159571690291136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGAAAAAAAAAAAAAAAA1954240.19047284505615344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCAAAAAAAAAAAAAAAA1760080.171548758149682No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGAAAAAAAAAAAAAAAA1757010.17124953613277394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTTGAAAAAAAAAAAAAAA1638110.15966077463102563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGGAAAAAAAAAAAAAAAA1623130.15820072713484237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAATGAAAAAAAAAAAAAAA1491860.14540630558451012No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAAAAAAAAAAAAAA1463680.14265970088207727No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAAAAAAAAAAAAAAAAA1455020.1418156413816135No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAAAAAAAAAAAAAAAAA1388410.13532340081280395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCAAAAAAAAAAAAAAAA1367550.1332902505611095No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1353820.13195203613369988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGAAAAAAAAAAAAAAAA1351880.13176295121096318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC1284560.12520150945909023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAAAAAAAAAAAAAAAAA1270470.12382820710787378No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT1265440.12333795083912867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACGAAAAAAAAAAAAAAAAA1246380.12148024020646826No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1209960.11793051191467958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTTGAAAAAAAAAAAAAAA1192100.11618976102804185No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1177290.11474628282921182No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTGAAAAAAAAAAAAAAA1157730.11283983896904196No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGGAAAAAAAAAAAAAAAA1140840.11119363054377257No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAAAAAAAAAAAAAAAAAA1140030.11111468271520725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA1118970.109062039172509No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC1116740.10884468897781685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTGAAAAAAAAAAAAAAAA1112960.10847626577784536No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAAAAAAAAAAAAAAAAA1060140.10332808762374658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT104689850.0104.375141
AGCAGTG107021550.0102.07032
AGTGGTA107291200.0101.821075
GTATCAA108198700.0101.041839
CAGTGGT108200900.0100.9426964
GGTATCA108844400.0100.360818
GCAGTGG109001750.0100.158993
GTGGTAT109822500.099.4730156
TGGTATC109841200.099.4367457
TTGTATC335750.033.5161257
GTTGTAT337650.033.3275266
CTAAGGT188550.032.857231
TGTATCA348500.032.310588
CAGTTGT374100.030.2932034
GCAGTTG375150.030.1084373
TGGTATA290050.028.917717
GGTATAA308850.027.0409058
AGCAGTT432650.026.3415432
ATGGGAT10121050.025.887925-29
ATGGGGG5785650.025.88132325-29
Produced by FastQC (version 0.11.5)