FastQCFastQC Report
Mon 20 Mar 2023
SRR6503017_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503017_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91584607
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA34442563.76073677970797No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8591520.9380965078552994No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7857220.8579192789460788No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA7804230.8521333721506279No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3244440.35425603780775083No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1351210.14753680168109473Illumina Single End PCR Primer 1 (100% over 21bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1314410.14351865920001164No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1049480.11459130899584469No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC958990.10471082766124662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1943000.0142.880221
TAGCGAC1957000.0141.959272
ATGAATC2711850.0141.53242
TATCAGC1919650.0141.33071
AGTTAGC1726900.0140.87682
ATCCGTC2171600.0140.229032
ATCAGCA1933650.0139.886372
GGCTAAC1917600.0138.916822
GATGTAC1903000.0138.635572
AGCGACT975850.0138.32793
GTCTGTC3254350.0138.151121
GATAGAC740700.0137.824941
GTCGTAG1137050.0137.80491
CCTAATC2056000.0137.60051
AGAGATC1753300.0137.199052
TCTGTCA3287000.0137.059312
TCACGCA1024450.0137.011752
ACACGAC2618750.0137.004551
CTGTCAT1651150.0136.971013
GCTAACT988050.0136.61993
Produced by FastQC (version 0.11.5)