FastQCFastQC Report
Mon 20 Mar 2023
SRR6503015_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503015_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93376812
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA54556135.842577919665966No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA34871083.734447477174526No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13953871.49436136243332No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13576551.4539530435029202No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3281790.35145663358050816No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2312160.24761607838999686Illumina Single End PCR Primer 1 (100% over 21bp)
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2272790.24339982821431086No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1805380.1933435037383799No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1699980.18205590484284256No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA1158070.12402115420260867No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC969750.10385340634674912No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA967710.10363493669070646Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC4867700.0141.373322
GATAGAC1396050.0140.742281
ATGAATC1616800.0140.698792
GATGTAC6677200.0140.489332
AGATGTA6671200.0140.48621
TCACGCA1776450.0140.157912
AGTTAGC1162750.0140.11532
TATCAGC1191750.0139.790331
ATTGGCG4295550.0139.716171
TAAGGTC1557600.0139.560242
ATAGACA1419250.0139.135962
ATCCGTC1561750.0138.98192
TGGAACA2735850.0138.698621
CGGATTG1888150.0138.471191
CACCTTA4964700.0138.413481
TTCACGC1803300.0138.406721
CAGATTC1387700.0137.883382
ATCATTC1805300.0137.363661
ATCAGCA1215300.0136.811862
CTAAGGT1587350.0136.789121
Produced by FastQC (version 0.11.5)