FastQCFastQC Report
Mon 20 Mar 2023
SRR6503014_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503014_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79876516
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC18277372.288203206058712No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7955620.9959898601486324No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT3499570.4381225140064947No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2107330.26382347472441087No Hit
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1615430.2022409189704769No Hit
CACTGTGTGACTGGAGTTCAGACGTGTGCTCTTCCCATGTACTCTGCGTT1243180.15563773462528085TruSeq Adapter, Index 5 (97% over 38bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC967770.12115826383814737No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT817770.10237927753383735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTCA1407750.0143.439061
CAATGGA7710900.0143.135311
ATGCCTA912500.0142.137631
CTCAATG2662050.0141.553631
ACAAGCT1050850.0141.511891
CAAGACT1258950.0141.141571
CATCAAG576400.0140.682481
ACGCTTA1096550.0140.355962
GAGTCAA1438200.0140.163072
CAAGCTA1066100.0139.967212
AGTCAAT829750.0139.137793
ACTTCGA632500.0139.05812
AACATCG1189500.0139.026842
AGTCACT455650.0138.866011
AAGACTA1278650.0138.818342
AACGTGA484100.0138.681461
AGTGGTC1106100.0138.522771
TCAATGA2700800.0138.180852
TGCCTAA941200.0138.134432
AGACTAT610100.0137.556823
Produced by FastQC (version 0.11.5)