FastQCFastQC Report
Mon 20 Mar 2023
SRR6503014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79876516
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3585413244.88695025206157No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21243822.659582698874848No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5671220.7099984180581937No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT3731180.4671185207927697No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3612090.4522092575995678No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3479300.43558484699057226No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3394980.4250285528227095No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2649440.33169198316060755No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2604050.32600946190492336No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2497130.31262380046721117No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA2401510.30065282266442367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA2158500.27022961291902114TruSeq Adapter, Index 2 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2157080.2700518385153403Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2155170.2698127194230655No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2064800.25849900614092886No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1981930.24812424217400766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA1961450.24556028457725926No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1904610.23844430070034603No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1873260.23451949256274524No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1867810.23383718939368864No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1866020.23361309349045717No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1781140.22298669110705832No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCAGTGGTATCAACGCAG1533360.19196630959717872No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1491960.18678330937718918No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCGG1459150.1826757191062264No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1436260.1798100457961887No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1379750.172735375689145No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA1328790.16635552807536072No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT1314180.16452645480932093No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1308080.16376277603294564No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC1296900.1623631155870644No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA1273440.15942608212907033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA1203050.15061372982266777No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1195040.14961093195401762No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATAAACGCAG1190210.14900624859501885No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1178770.14757403790621013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT1146600.14354657131014578No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1127640.141172907441281No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA1120470.14027527189593497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGGTGGTATCAACGCAG1108790.13881301482903938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1108370.1387604336673873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1105540.13840613679244598No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG1069020.13383407959355664No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTGTCAACGCAG1048360.131247587213243No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG1031120.12908925572066762No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTG1020570.12776846701726452No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG989620.12389373617647519No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAG964240.12071633169378594No Hit
AAGCAGTGGTATAAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG905640.11338000771090216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG836880.10477172038900644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCAGTGGTATCAACGCAG826220.10343716042898014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGAAG800920.10026977140565319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT198228900.043.4556271
GTATCAA205944400.041.800329
AGCAGTG206554100.041.6693572
AGTGGTA207335800.041.539655
GGTATCA210867800.040.7624638
CAGTGGT216098200.039.834374
GCAGTGG216259650.039.7858923
TGGTATC216767900.039.6556977
GTGGTAT217727900.039.559226
ACACTCG155100.021.731830-34
GGTATAA1078450.020.0114238
TGGTATA1085900.019.9205347
AGTTGTA783800.019.0592695
AGCAGTT789000.018.9799352
GTATAAA1131250.018.8737819
ACCTAGC136550.018.83138735-39
CGTCAAG134800.018.7897695-99
TGGAGTT212250.018.7573226
CTAGTCC176950.018.55279770-74
GATAAGT180700.018.4873795-99
Produced by FastQC (version 0.11.5)