FastQCFastQC Report
Mon 20 Mar 2023
SRR6503013_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503013_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82837614
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA27987873.378642702094244No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA6200290.7484872753577861No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4440690.5360716932286341No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2973210.35892028469096177No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1435150.17324859211903423Illumina Single End PCR Primer 1 (100% over 21bp)
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1303200.15731983782149978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1743650.0142.9451
TAGCGAC1756850.0142.287872
GATAGAC1160600.0141.451891
ATCCGTC1968950.0140.719512
GAACAGG7803100.0140.392011
GATGTAC2120350.0140.052192
AACAGGC7832600.0139.991522
ATGAATC1558450.0139.890212
TAAGGTC2415100.0139.845382
TATCAGC1120500.0139.797151
AGTTAGC1038650.0139.505312
ACAGGCT3145050.0139.356633
CAGATTC1901550.0139.14812
GTACGCA917750.0139.03681
AGCGACT861650.0138.85813
AGAGATC2150150.0138.72742
GGCTAAC1618550.0138.464312
CGGATTG1960350.0138.063521
ATCAGCA1131800.0137.968552
GCGAGTA1325200.0137.928151
Produced by FastQC (version 0.11.5)