FastQCFastQC Report
Mon 20 Mar 2023
SRR6503010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47288673
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG577803512.218644832769995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA7316601.5472204094202433No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4463400.9438623917401954No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3067210.648614098348668No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3024400.6395611904778973No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2338200.4944524453033393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1483290.313667080486695No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1306220.2762225956308818No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA1153830.24399711956391754No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA991600.20969080692960024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC984900.2082739771530489No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC795910.1683088040977593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG773970.1636692152473807No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA721210.15251220942486587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA682210.14426499132255202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT658800.13931454578985542No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG612760.1295785990865085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAAAAAAAAAAAAAAAAA491170.10386631064906388No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT477350.10094383490101319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT50204800.076.598321
AGTGGTA52091450.074.1802755
GTATCAA52251650.073.999339
AGCAGTG52113850.073.884962
CAGTGGT52697450.073.326724
GGTATCA52766400.073.250168
TGGTATC53295600.072.524317
GTGGTAT53306150.072.4869466
GCAGTGG53574350.072.09633
ATGGGGG2706950.024.00580625-29
ATGGGGC2179550.023.99443425-29
ATGGGGT1704350.023.64028425-29
CGATAGG89950.023.25815835-39
CATGGGG9778100.023.23976925-29
AAACCGC565200.022.72546685-89
GAAACCG574600.022.65445185-89
ATGGGAT2649050.022.53326825-29
CGACCTA110750.022.2434145-49
TACGCAC111500.022.19711750-54
GATAAGT600750.022.02824895-99
Produced by FastQC (version 0.11.5)