FastQCFastQC Report
Mon 20 Mar 2023
SRR6503009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41120204
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG932697822.68222696560552No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8312112.0214175007497532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3589830.8730088012209277No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1774000.43141809315926544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1442550.3508129483015211No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1128970.27455359900451853No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1128660.2744782102734704No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1049650.2552638114343985No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1021220.24834993522892057No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA821660.19981904759032812No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC821570.19979716053937865No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT820530.19954424350618494No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT775900.188690698129805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG735940.1789728475082468No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC687410.16717086325739045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA580880.14126389061688507No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT575650.13999200976726672No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG549710.1336836753047237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC533600.12976589318477116No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC520310.1265339053279016No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA500640.1217503687481706No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG488870.11888802886289183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG461060.11212493011951011No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG457170.11117892313958365No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG430800.10476601721139323No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA428770.1042723426177555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT64687350.053.784731
AGCAGTG66105900.052.6066132
GTATCAA66273650.052.5071959
AGTGGTA66229400.052.4992145
GGTATCA66934550.051.967678
CAGTGGT67170050.051.7644424
TGGTATC67532300.051.5045937
GTGGTAT67554950.051.467916
GCAGTGG67998850.051.1121523
CGATAGG88400.023.4881235-39
ATGGGGC1726550.022.95617525-29
ATGGGGG2026850.022.84870325-29
TGGAGTT315800.022.6634046
CATGGGG6555000.022.46160325-29
CGACCTA87350.021.75960245-49
ATGGGGT1164500.021.1544425-29
ACCTAGC278550.020.8684835-39
ATAGGCG266950.020.740446100-104
TACGCAC91450.020.7066150-54
ACCTTAC281150.020.61803455-59
Produced by FastQC (version 0.11.5)