FastQCFastQC Report
Mon 20 Mar 2023
SRR6503008_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503008_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60612624
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA21320623.5175213665060925No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6856361.131176898066647No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA6066491.000862460598967No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4566130.7533298673886812No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2157790.3559967969708752No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATA1263780.20850112016269087No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA858510.14163881108331494Illumina Single End PCR Primer 1 (100% over 21bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC776340.1280822292069058No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA647610.10684407921359748No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC637560.1051860087759936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC1416400.0142.167241
TGGAACA2428800.0141.226331
TATCAGC1310250.0141.099441
GTGCGAA1852900.0140.859992
ATAGCGA689350.0140.7861
GCCACAT1935350.0140.684981
ATAGACA1443650.0140.60612
ATCAGCA1319000.0140.42112
ATGAATC891600.0140.38972
GGCTAAC911650.0140.29552
GGAACAA2461050.0139.440572
TCACGCA1394050.0139.395232
TAGCGAC694000.0139.203932
ACATCGG2690650.0138.817521
CGAGTAA1007450.0138.704472
TGCGAAT917300.0138.704063
AGAGATC1394100.0138.563842
GCGAGTA1008550.0138.455721
CATCGGT1924600.0137.939362
CCACATA1984800.0137.688082
Produced by FastQC (version 0.11.5)