FastQCFastQC Report
Mon 20 Mar 2023
SRR6503007_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503007_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80515068
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10840761.3464262366393331No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4744100.5892189024792229No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1697640.21084749006235703No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1460830.1814356040784813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC1976550.0144.386171
AGTCACT2332100.0144.063431
AGAGTCA1830800.0143.828281
AACGTGA2174900.0143.52851
CGCATAC1779750.0142.589341
GTCACTA2393550.0141.928382
CCGAAGT1398200.0141.908021
ACTTCGA1564600.0141.82872
ACGTGAT2234050.0141.602482
CGAACTT1192350.0141.595921
CTCAATG1822450.0141.495381
GAGTCAA1879500.0141.13682
CAGATCT1485650.0140.998321
ACGCTTA1386350.0140.950932
AACATCG2123550.0140.938052
AGTGGTC1693850.0140.663251
CCGTGAG1545450.0140.501721
ATGCCTA650800.0140.433361
CAATGGA3142650.0140.315611
CACTTCG1590550.0140.143231
Produced by FastQC (version 0.11.5)