FastQCFastQC Report
Mon 20 Mar 2023
SRR6503006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66781171
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1530333722.91564638781192No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA9445101.4143357863551091No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2812360.4211306806824337No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2440650.3654697818940611No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1835960.2749218039318298No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1385500.2074686590925457No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1344650.2013516654267713No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1319290.19755418784136025No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1316850.19718881539229077No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1305960.19555811622410751No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGATCAAGTGAATAAGTGCA1185190.17747367742323655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC1181180.1768732087671838No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1144160.1713297300522029No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG1081960.1620157274570702No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC991310.14844154200290977No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT975070.14600971881729957No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC890700.13337591819107214No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG878480.13154606108958466No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA845970.12667792243415438No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG841510.12601006951495355No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC837470.12540510857469092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAAGCGACTAAGTGCATGT819780.12275615831893692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA814050.12189813203485156No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG779800.11676944089524875No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG744800.11152844265039917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT740290.11085310259084855No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA736980.11035745389969277No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCGGTGGCTCACGCTTG706380.10577532400562427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCACGCTT700530.10489932858469943No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA688090.10303652806567289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT95820200.058.2038961
GTATCAA99673550.056.004999
AGTGGTA99817250.055.858235
AGCAGTG100266900.055.6173362
GGTATCA101974800.054.726248
CAGTGGT103151350.054.0506364
TGGTATC104465150.053.4125947
GTGGTAT104529000.053.354426
GCAGTGG104548700.053.3051953
GACTGGA428750.038.9107553
TGACTGG433350.038.5316732
GTGACTG451300.037.1060451
TGGAGTT559000.029.6607486
AGTTCAG596050.027.8282019
ACTGGAG641300.026.058714
CTGGAGT701950.023.9281185
CGATAGG252700.022.97392835-39
ATGGGGC3355500.022.89044225-29
ATGGGGG4837000.021.91781425-29
TGCGTAA222250.021.78377595-99
Produced by FastQC (version 0.11.5)