FastQCFastQC Report
Mon 20 Mar 2023
SRR6503005_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503005_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72103934
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11698641.6224690319948423No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3668240.5087433925588581No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1462000.2027628617323432No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1312350.1820080995858007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT4725200.0144.409741
CGCATAC3326350.0143.417021
CGACTGG5057750.0142.833821
GAACTTA4810650.0142.00382
ACGTATC918350.0141.671451
AGAGTCA958800.0141.543691
CCGTGAG1735250.0140.792971
GCATACA3386050.0140.751622
GAGCTGA1389700.0140.679891
AACGTGA878150.0140.48171
ACTTCGA1279300.0140.472752
CAATGGA3392500.0139.983411
CGTGAGA1744650.0139.703052
GTCACTA1066200.0139.702042
ACGTGAT898800.0138.937012
ACTGGAT2629900.0138.547933
GAGTCAA976100.0138.321812
ACGCTTA814850.0137.794222
AGCTGAA1412150.0137.747212
CTCAATG1279700.0137.548221
Produced by FastQC (version 0.11.5)