FastQCFastQC Report
Mon 20 Mar 2023
SRR6503004_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6503004_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103723069
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA84002928.098769233293703No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12698141.224234890311624No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA8936100.8615344769638468No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8095960.7805361023399723No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC7046180.6793262162345003No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6425030.619440791903294No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA4899750.4723876807000379Illumina Single End PCR Primer 1 (100% over 21bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC4510020.4348135900221001No Hit
CCATGTACTCTGCGTTGATAGCACTGCTTCCCATGTACTCTGCGTTGATA4148490.3999582773625798No Hit
CCATGTACTCTGCGTTGATACCAGTGCTTCCCATGTACTCTGCGTTGATA3008810.29008108119130177No Hit
CCATGTACTCTGCGTTGATAGCAGTGCTTCCCATGTACTCTGCGTTGATA2647750.2552710814987551No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGCTA2054940.19811793266549027No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1521500.14668867925610646No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1377350.13279109587472773No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1150330.1109039687207867No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT1081470.10426513700631053No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCTTTGATA1079320.10405785428504818No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAATC2654550.0141.43862
TGGAACA3209650.0140.905241
AGTTAGC1570300.0140.264242
AACTCAC2290700.0140.159711
AGAGATC6008000.0139.459412
CCTAATC903750.0138.465561
TCACGCA2249150.0138.301712
TACGCAA1158900.0137.335572
GGAACAA3284750.0137.112322
GCCACAT1770200.0136.757131
GAGTTAG1620500.0136.58621
AAGGACA1662900.0135.933881
TTCACGC2301350.0135.80711
ACGTGAC2490300.0135.566825
TACGTGA2424900.0135.489584
GGCTAAC587750.0135.40232
AGATGTA2615600.0135.289731
TAAGGTC768600.0135.116532
AGCCATG1135700.0134.762331
CTAATCC924650.0134.495272
Produced by FastQC (version 0.11.5)